Development of Data-driven Machine Learning Models and their Potential Role in Predicting Dengue outbreak.

ABSTRACT: Dengue fever is one of the most widespread vector-borne viral infections in the world, resulting in increased socio-economic burdens. The WHO has reported that 2.5 billion people are infected with dengue fever across the world, resulting in high mortalities in tropical and subtropical regions. The current article endeavors to present an overview of predicting dengue outbreaks through data-based machine-learning models. This artificial intelligence model uses real-world data such as dengue surveillance, climatic variables, and epidemiological data and combines big data with machine learning algorithms to forecast dengue. Monitoring and predicting dengue incidences have been significantly enhanced through innovative approaches. This involves gathering data on various climatic factors, including temperature, rainfall, relative humidity, and wind speed, along with monthly records of dengue cases. The study functions as an efficient warning system, enabling the anticipation of dengue outbreaks. This early warning system not only alerts communities but also aids relevant authorities in implementing crucial preventive measures.

Synthesis of Cinnamum zeylanicum and Acacia nilotica Extracts and Their Antibacterial Activity against Staphylococcus aureus and Streptococcus pyogenes.

Different plants are used medically and thofese therapeutic plants have great importance for healing contagious wounds. This herbal treatment is actually also a substitute of different antibiotics and having less side effects on intestinal systems of animals. The foremost concern of this study was to observe the antibacterial activity of Cinnamum zeylanicum and Acacia nilotica. Pathogenic bacteria obtained from wound samples and later identified by biochemical and molecular characterization. Methanol (an organic solvent) was used to extract Cinnamum zeylanicum and Acacia nilotica to check their antimicrobial exertion by using agar diffusion method. Different antibiotics such as, ampicillin, oflaxocin, ticarcillin and cefexime, showed their susceptibility toward antibiotics. The zone of inhibitions for antibiotic and plant extracts' antibacterial activity were measured. Pathogenic bacteria were identified as Staphylococcus aureus and Streptococcus pyogenesby molecular characterization. These bacteria showed susceptibility to antibiotics and also the plant extracts. Antibiotic oflaxocin showed maximum activity against these two pathogens (12.25 ± 0.44 and 12.375 ± 0.47) while antibiotic cefixime showed minimum effect (1.25 ± 0.28 and 0.625 ± 0.25). Plant extracts showed significant antibacterial activity with maximum activity (14 ± 0.9 by Acacia nilotica and 12 ± 0.5 by Cinnamum zeylanicum) in 100% solution. It can be concluded thatmethanolic extract of traditional therapeutic plants proved to be a promising source of antimicrobial agents against antibiotic resistant bacteria. Cinnamum zeylanicum and Acacia nilotica were observed to be competent as antibacterial tool against pathogenic bacterial strains.

Bacteriocin-mediated inhibition of some common pathogens by wild and mutant Lactobacillus species and in vitro amplification of bacteriocin encoding genes.

Lactobacilli are the most common probiotics used in food and other industries because of their capability of producing bacteriocins. Bacteriocins are compounds that are used to kill pathogenic microorganisms. As most bacteria have become resistant to synthetic antibacterial tools, the importance of using probiotics as antibacterial agents has increased. This work was done to check the bacteriocin effect on some common pathogens and the influence of mutation on the bacteriocin activity of Lactobacilli was also investigated. Four strains were isolated, identified from meat and pickles samples via culturing methods, staining, biochemical tests, and ribotyping. Preliminary tests, including Gram staining and catalase test, were done for the confirmation of Lactobacillus species. All strains were gram-positive and catalase-negative. Antibacterial activity was checked against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus thuringiensis, Escherichia coli, and Salmonella enteritis via agar well diffusion method. The mutations were done using ethidium bromide and the influence of wild and mutants were also checked. Interestingly, mutants developed more virulence than wild ones. It was also observed that they all were sensitive to pepsin. Protein estimation was done via Bradford method. Ribotyping of GCU-W-PS1 revealed 99 % homology with Lactobacillus plantarum and GCU-W-MS1 to Lactobacillus curvatus (99 % homology). Curvacin A, sakacin P, and plantaricin A genes were also amplified using specific primers. Gene sequence showed the presence of curvacin A gene in GCU-W-MS1. It was concluded that lactic acid bacteria could be used as antibacterial tools against common pathogens.

Bacteriocin Production by Lactobacilli and Their Role as Antibacterial Tool against Common Pathogens.

Lactobacilli are commonly found in raw and fermented foods. These bacteria are of common interest because of their capability of bacteriocin production. Bacteriocins are compounds that may be used as natural preservatives in place of chemical additives. In the present study five different samples of poultry, meat and pickle were used to isolate lactic acid bacteria (LAB) by using the MRS culture medium. Antibacterial activity was checked against Pseudomonas aeruginosa, E. coli and Staphylococcus aureus. Five strains from the poultry meat and six strains from pickle had shown antibacterial activity out of eight strains against pathogenic bacteria. Three strains of poultry meat i.e. M3, M7, M11, had shown antibacterial activity against all three indicator strains. While one strain M1 was active against Pseudomonas aeruginosa and E. coli but it was found not to be active against Staphylococcus aureus. E. coli and Staphylococcus aureus were not susceptible to M2. In the case of pickle strains H1, H2, NAT1, NAT2, were found to be active against Pseudomonas aeruginosa and E. coli but Staphylococcus aureus was not susceptible to bacteriocins of these strains. NAT3 and SHZa strains showed antibacterial activity against all the three used pathogens. The highest activity (6 mm) had shown by the lNAT3 strain of pickle against Pseudomonas aeruginosa. The least antimicrobial activity had shown by SHZa strain. M3 had an inhibition zone of 5 mm against Pseudomonas aeruginosa. M11 had an inhibition zone of 5 mm against E. coli. Bacteriocins of all isolates were sensitive towards enzyme pepsin showing their proteinaceous nature. Detection of the bacteriocin gene by PCR the strains M2, M7 were amplified for Curvacin and plantaricin genes. From current study, it is concluded that different species of Lactobacilli can be used as antibacterial tool against common bacterial pathogens.

Effect of medicinal plants, Heavy metals and antibiotics against pathogenic bacteria isolated from raw, Boiled and pasteurized milk.

Present study has been undertaken to isolate and identify the bacterial flora in raw, boiled and pasteurized milk. Agar disc diffusion method was used to determine their sensitivity using medicinal plants, antibiotics and heavy metals. Methylene blue reduction test was used to test the quality of milk samples. Total 10 pathogenic strains were isolated, five strains were isolated from raw milk, three from boiled milk and 2 two from pasteurized milk. To determine optimum conditions for growth, these pathogenic microorganisms were incubated at various temperatures and pH. Gram's staining and biochemical tests revealed that these pathogenic bacteria include Lactobacillus sp., E. coli, Salmonella sp., Pseudomonas sp., Streptococcus sp. and Staphylococcus. Ribotyping revealed S2 as Pseudomonas fluorescens, S5 as Lactococcus lactis and S9 as Lactobacillus acidophilus. Prevalence of pathogenic organisms provided the evidence that contamination of milk arises during milking, transportation and storage of milk. Raw milk is more contaminated than other two types of milk because it contains highest percentage of pathogenic organisms and pasteurized milk was found to be of best quality among three types. So it is recommended to drink milk after proper boiling or pasteurization. Proper pasteurization and hygienic packing of milk is essential to minimize contamination in milk which can save human beings from many milk borne diseases. Our study suggests that antimicrobial use in animal husbandry should be minimized to reduce the hazard of antibiotic resistance. Plant extracts are better alternative against pathogenic bacteria in milk.

Antibacterial activity of different plant extracts and antibiotics on pathogenic bacterial isolates from wheat field water.

The present work was carried out to study bacterial pathogens isolated from wheat field water and also effect of some plant extracts on these bacterial pathogens. Five bacterial strains were isolated from wheat field water. Different morphological and biochemical tests were performed to identify and characterize bacterial pathogens. Among isolated strains two belonged to genus Staphylococcus sp., other two were Pseudomonas spp. and one strain belonged to genus Salmonella sp. Effect of various parameters such as temperature, pH, antibiotics and heavy metals of these pathogens were also studied. Optimum temperature for all bacterial strains was 37oC and optimum pH was 7 except strain 3 which had pH 6. Different antibiotics with different potency were applied to check the resistance of bacterial strains against them. Among these antibiotics Cloxacillin and Teicoplanin were most potent while Oxacillin was as less potent antibiotic because three bacterial strains were resistant against it. While remaining antibiotics proved as potent. Seven heavy metals which were zinc (Zn+2), copper (Cu+2), Ferrous (Fe+2), mercury (Hg+2), Nickel (Ni+2), chromium (Cr+2) and cobalt (Co+2) with different concentrations were applied to bacterial strains. Minimum inhibitory concentration of heavy metals for all bacterial was different. Different plant extracts (Artemezia incise, Colebrookia oppositifolia, Rhynchosia pseudocajan) checked for their antibacterial activity against these pathogens. These plant extracts showed antibacterial activity against antibiotic and metal resistant bacterial isolates.